Prevalence and Antibiogram of Salmonella Species Isolated from Clinical Samples and Their Effects on the Leucocyte Count of Albino Wister Rats (Rattus Norvegicus)
PREVALENCE AND ANTIBIOGRAM OF SALMONELLA SPECIES ISOLATED FROM CLINICAL SAMPLES AND THEIR EFFECTS ON THE LEUCOCYTE COUNT OF ALBINO WISTER RATS
( Rattus norvegicus).
- S. Udoekong1, A. N. Umo2 and A. A. Ibuot1
1 Department of Science Technology, Akwa Ibom State Polytechnic,
Ikot Osurua, Ikot Ekpene.
2 Department of Medical Microbiology and Parasitology,
College of Health Sciences, University of Uyo, Uyo.
This study examines the prevalence and antibiogram of Salmonella species isolated from human stool samples and their effects on experimental rats in two hospitals in Akwa Ibom State using standard procedures. The bacteriology of the samples was carried out following the method of ISO 6579:2003 for Salmonella isolation; while the Kirby-Bauer disk diffusion method was used to assay the antibiotic susceptibility of the isolate. The Salmonella species isolated from stool samples showed high resistance to amoxicilin (83.33%), followed by tetracycline and augmentin (70%), cotrimoxazole and gentamicin (66.66%), ofloxacin (22.77%), nalidixic acid (24.44%) and nitrofurantoin (20%). There was no significant difference (P> 0.05) in the resistance patterns of isolates from the two hospitals. However, there is significant effect (p<0.05) of the oral challenge of the cell suspension on the leucocyte count of the neonatal rats which is indicative of the pathogenicity of the Salmonella spp. The high prevalence of antibiotic resistant species and the biological effects elicited by the cell suspension from Salmonella spp. on the experimental rats is of public health significance.
Keywords: Salmonella spp., antibiogram, leucocyte count, neonatal, cell suspension.